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Kynurenine is subsequently converted to NAD + in a chain of reactions with the rate-limiting step by quinolinic phosphoribosyltransferase (QPRT), which converts quinolinic acid into nicotinic acid mononucleotide (NAMN) 26 , finally entering the PreissHandler pathway

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The lysates were incubated with anti-Flag M2 agarose (Sigma, A2220) or protein G beads immobilized with anti-SMC3 antibody (1 g) at 4 C for 2 h

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