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Biomarker analyses reveal systematic endocrine disruption, characterized by 28.5% testosterone suppression and 45.2% FSH elevation in exposed populations, indicating primary testicular dysfunction rather than hypothalamic-pituitary axis impairment

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The integrated GHK standards (0.1, 0.2, 0.5, 0.75, 1.0, and 2.0 mg/mL) and recombinant GHK peptide peak areas (l = 254 nm) for the different time points were used to monitor the changes in concentration over time

doi: 10.3390/biom12111622

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PCR amplification targeted the 3-adrenergic receptor (3-AR) gene and -actin as a housekeeping control