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Then, 95 L supernatant was transferred to a labeled 1.5-mL microcentrifuge tube, and 5 L of an internal standard of Sph/Cer was added

however, these neutrophils upregulate the expression of adhesion proteins and enhance the cytotoxic response (82)

Treatments in these co-cultures included PBS as a control, FOXO4-DRI at 50 M, hydrogen peroxide (H2O2) at 50 M to induce senescence, and a combination of H2O2 with FOXO4-DRI