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To reduce potential batch effects, samples from healthy controls and clinically suspected patients were randomized and analyzed across multiple batches

These protective effects are mediated through antioxidant properties, enhancement of blood flow and oxygen delivery, and modulation of inflammatory cytokine production

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Obesity treatment guidelines emphasize a stepwise approach, focusing initially on evidence-based nutrition and lifestyle modifications centred on hypocaloric dietary patterns and physical activity regimens, tailored to individual needs, followed by escalation to pharmacotherapy and bariatric surgery [[4], [5], [6], [7]]

In this study, composite dietary fiber supplementation significantly elevated levels of butyric acid, valeric acid, and total SCFAs in colonic contents, along with a marked increase in serum butyrate, indicating enhanced SCFA production by core SCFA-producing microbiota and systemic metabolic improvement

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