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When a cell over-expresses NNMT, it does two costly things at once: it burns through methyl-donor capacity, and it diverts nicotinamide away from NAD+ regeneration into an inert metabolite (1-methylnicotinamide) that gets excreted

MYCN-enhanced oxidative and glycolytic metabolism reveals vulnerabilities for targeting neuroblastoma

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Stress management: Chronic stress can alter methylation dynamics

I'll post back about the process, fingers crossed

The most methodologically sound approach is staggered introduction: start MOTS-c alone for 1014 days to establish baseline metabolic response, then add 5-Amino-1MQ at starting dose and monitor for synergistic effects over the following 1421 days