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Concentrations decline sharply with age: plasma levels drop from roughly 200 ng/mL at age 20 to under 80 ng/mL by age 60

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The GSH-Glo Glutathione Assay GSH Depletion and Recovery in Treated Cells HeLa cells (5000 cells/well in 96 well format) were exposed to l-Buthionine-sulfoximine (BSO)