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In preclinical models of glaucoma and DR, GLP-1RAs have demonstrated robust neuroprotective effects in ocular tissues, primarily by modulating oxidative stress, inflammation, mitochondrial function, and synaptic plasticity [42, 43]

For GSH assay, cells were treated in Earles balanced salt solution (EBSS), supplemented with 25 mM glucose, 4 mM glutamine, 1 X MEM vitamin solution (Life technologies, CA, USA), 0.22% wt/vol NaHCO 3 , 1 mM sodium pyruvate, 0.2% BSA, 25 mM HEPES, 100 U/ml penicillin and 100 g/ml streptomycin in the presence or absence of 1 X MEM amino acid solution (Gibco, CA, USA) with or without 100 M cystine

[25] In addition, calcium and DAG together work to activate protein kinase C, which goes on to phosphorylate other molecules, leading to altered cellular activity

doi: 10.1159/000512806

The topical GHK-Cu delivers all the copper that matters for the local effect