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According to the manufacturers instructions, serum levels of thyroid hormones and sex hormones were measured using an ELISA kit (Bim, USA)

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The activity of glutathione-S-transferase (GST) in the brain homogenate was measured using the procedure outlined by Habig et al

At OD 600 of 0.5, the culture was chilled in an ice-water bath for 5 min and incubated at 15C for 45 min on a shaker

The intestines were flushed with about 40 mL of cold PBS to remove its contents