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Supplied lyophilized requires reconstitution with a suitable sterile solvent before research use

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Following the electrophysiological testing, histology testing took place by initially collecting a 10-mm long piece of the spinal column and the surrounding muscle

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Briefly, single stranded oligodeoxynucleotide (ssODN) templates were used to introduce LoxP and BamHI restriction enzyme sites between exons 3 and 4, and exons 7 and 8 of the Thbs1 gene by homologous directed repair at the position of the double strand break (DSB) induced by Cas9 nuclease

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