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References: He L, Feng D, Guo H, Zhou Y, Li Z, Zhang K, Zhang W, Wang S, Wang Z, Hao Q, Zhang C, Gao Y, Gu J, Zhang Y, Li W, Li M

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After blocking in 5% FBS in PBT for 1 h at RT, brains were incubated with the primary antibody (mouse anti-Su(var)2-10 (Andreev et al, 2022), 1:100

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BPC-157: More localized, especially in the GI tract and connective tissue TB-500: Broader systemic effects, influencing cell migration and circulation In some cases, both may be considered together (bpc157 and tb500) as complementary, though decisions must be individualized and physician-guided

The key factors are the date of the first puncture and proper handling, not the volume of the vial