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The pH of each sample was adjusted within the range of 7 to 9 using 014 pH strips (#13640516, Thermo Fisher Scientific) before subjecting them to digestion with mass spectrometry-graded trypsin (#90057, Thermo Fisher Scientific) at a 1:20 trypsin to protein (w/w) ratio

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This increases oxidative deamination of amino acids, generating ammonium, which is mostly transformed into urea ( Madsen, 1983 )

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