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For gene ontology (GO) analysis, the Seurats FindMarkers output for the specified groups was used to plot gene ontology terms with the barcodeplot functions from limma 131

Blots were subsequently washed three times with 1X TBST for 5 mins each and developed using a commercial chemiluminescent reagent (Clarity, Bio-Rad-1705061) and imaged using the ChemiDoc MP (Bio-Rad)

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doi: 10.1136/jitc-2020-002189 84 BozorgmehrNSyedHMashhouriSWalkerJElahiS