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After transduction and selection using puromycin, colonies grown from single cell were picked and expanded for experiments

As a defined small molecule (not a peptide), it has different handling considerations than the rest of the Durham Peptides catalog, but the research utility is in the same conceptual category as NAD+-pathway research compounds

Equal amounts of protein lysates were loaded on Novex WedgeWell Tris-glycine or Bis-Tris gels (ThermoFisher) and blotted onto nitrocellulose or PVDF Trans-Blot Turbo membrane (0.2 m, Bio-Rad) according to standard protocols

consider separating oral NAC from these antibiotics by at least two hours

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