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Most human trials primarily assess glycemic control, body weight, and lipid parameters rather than GLP-1 directly, so proposed incretin mechanisms often come from preclinical work

Acetylcholine has been well studied for its ability to regulate mucus levels in the intestinal lumen by inducing mucus secretion in goblet cells, regulating microbial populations [296,297,298]

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After stabilized in Krebs buffer for 30 min, aortic rings were depolarized with potassium chloride (60 mmol/L) for evaluation of their maximal contraction

(PubMed) Harris JC, Cottrell SL, Plummer S, Lloyd D

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