JA and JA-Ile quantification in leaf tissues Metabolite extraction and quantification of JA and JA-Ile in leaf tissues were performed according to previously described methods using AB Sciex 4500 QTRAP triple quadrupole mass spectrometer (AB SCIEX, MA, USA) equipped with an ACQUITY UPLC BEH C18 column (Waters, Eschborn, Germany) (50 2.1 mm, 1.7 m) 45
The released cytochrome c interacts with other proteins in the cytosol and activates caspases, resulting in cell apoptosis (Suen et al., 2008)
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The authors acknowledge the support of the Mayo Clinic General Clinical Research Center (DK TR000135)