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Ind J Clin Biochem

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cDNA synthesis and PCR amplification Complementary DNAs (cDNAs) were synthesized at 42 C for 60 minutes with 3 L of RNA solution and 200 U/ L of Superscript II enzyme in a 20 L reaction mixture containing 0.15 g/ L random primers, first strand buffer 1X, 10 mM dithiothreitol (DTT), 0.5 mM dNTPs, and 2 U/ L RNAse inhibitor, then incubated at 94 C for 5 minutes