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The present authors are by no means recommending any lack of caution about use of antioxidants

Bibcode:2020ChRv..120.4707F

Checking the dosage unit calculations and the 50-unit conversion can help verify correct dosing if results seem delayed

In reaction mixture without enzyme solution, varying amounts of glutathione (0-300 l of stock solution giving 0-260 M final glutathione concentrations) were reacted with 150 l of monobromobimane solution (final concentration 50 M) in presence of phosphate buffer (3.0 ml) in cuvettes

it is a fundamental necessity dictated by the molecule's biochemistry

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