Absorption, distribution, metabolism and excretion of apigenin and its glycosides in healthy male adults
Logarithmic growth phase cells were inoculated in 6-well plates at a density of 350,000/well and transfected after 24 h
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Mounting: Dehydrate through a graded ethanol series (70%-80%-95%-100%), replace the dehydrant in the tissue with an organic solvent such as xylene to render the tissue transparent, then mount the sections with a fluorescent mounting medium such as Acrymount or DPX