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To the plasma samples (250 L), 50 L of water, 50 L of internal standard solution (buprenorphine-d4 40 ng/mL) and 250 L of sodium carbonate buffer (0.05 M
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PMCID: PMC8398778
Furthermore, a recent clinical study in women with obesity treated with semaglutide showed improved recognition of basic tastes, altered gene expression in the tongue (measured by tongue biopsy), decreased activation of putamen in response to calorie-dense sweet and savory food cues, and increased cortical brain activity in response to the sweet taste stimulus compared to placebo