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Phase contrast images, analysed using PHANTAST in Fiji, were used to determine the cell containing region, and BaSiC was used for illumination correction of fluorescent images

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To determine the effect of the V229A substitution on the overall GLP-1R binding to cholesterol in active versus inactive conditions, cells from both sublines were labelled with PhotoClick cholesterol and exposed to UV light prior to SNAP/FLAG-hGLP-1R purification by anti-FLAG immunoprecipitation and click chemistry to fluorescently label covalently bound PhotoClick cholesterol (Figure 3C)

Glutathione is a powerful antioxidant that consists of three amino acids: Cysteine, Glutamic Acid, & Glycine

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