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Then, the high-sensitivity RT-qPCR reaction was measured using the SYBR green chimeric fluorescence method (Accurate Biology) and detected by CFX96 Real-Time PCR System (Bio-Rad, Hercules, CA, United States)

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However, this cannot exclude the possibility of ATP synthesis defect in patients with CFS as insufficient ATP synthesis could be offset by compensatory homeostatic mechanisms

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PMID 20043075