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coli culture was collected by centrifugation for 10 min at 3500 rpm and 4 C, washed with ice-cold PBS at pH 7.3, and lysed in 25 ml of lysis buffer (PBS, pH 7.3

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Immunofluorescence analysis showed that GSH treatment caused glomerular-like structures to be organized in a pattern similar to that in WT kidney organoids, while the glomerular-like structures were linear and unorganized in Fabry kidney organoids (Fig

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