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The FDA isnt required to follow the groups advice, though it usually does

These reactions are typically temporary and resolve on their own within a few days

Note that some multidose HCG products include a preserved diluent containing benzyl alcohol - this type of product also requires special caution in infants

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After different groups of HUVECs are treated accordingly, they are seeded into 24-well plates pre-coated with 150 L/well growth factor-reduced Matrigel (Corning, catalog number 354234) and incubated at 37 C in a cell culture incubator for 12 h

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