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In addition, thyroid hormones affect mitochondrial function, energy expenditure and thermoregulation and are therefore essential for metabolic homeostasis [4]

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Materials and methods: Oral glucose tolerance test samples (0, 30, 60 and 120 minutes) were randomly selected from an extensively phenotyped prediabetes cohort

After incubation, 1 mL of absolute EtOH was added and SP3 beads were magnetically captured and washed three times with 80% EtOH, then resuspended in 200 L 50 mM HEPES buffer (pH 7.4) containing 1 mM TCEP and incubated for 3 min at RT

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