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After 48 h, FLAG-tagged Sirt7 (WT) and the corresponding mutants (DM, CA) were collected and lysed in lysis buffer (50 mM Tris-HCl (pH 7.5), 300 mM NaCl, 1% Nonidet P-40, 1 mM EDTA, 10% glycerol and 1 mM DTT) supplemented with protease inhibitors (Roche, cat

In a randomized, placebo-controlled, cross-over study involving 34 patients (28 idiopathic, six diabetic) with gastroparesis, patients received prucalopride, 2 mg once daily, or placebo for 4 weeks, with a 2-weeks washout between treatments