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examined the effectiveness of a Japanese oral supplement containing collagen peptides on skin hydration and the natural moisturizing factor content in 99 healthy Japanese women, aged 3550 years, who were divided into three groups (33 subjects/group) and received 1 or 5 g of collagen peptides or placebo once daily for 12 weeks [38]

It has been reported that IGFBP-3 binds to a nuclear receptor, 9-cis retinoic acid receptor-alpha (RXR-), which interacts with peroxisome proliferator activated receptor-gamma (PPAR-), a nuclear protein involved in the regulation of glucose and lipid metabolism [175, 176]

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Firefly luciferase activity was measured and normalised to the control Renilla luciferase activity

IGF-1 is the readout