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We show that the PfGrx-catalyzed oxidation of roGFP2 occurs via a monothiol mechanism and is up to three orders of magnitude faster when roGFP2 and PfGrx are fused

Discard the vial and reconstitute a fresh one using slower injection technique and proper sterile handling

[DOI] [PubMed] [Google Scholar] Urata Y, Yamamoto H, Goto S, Tsushima H, Akazawa S, Yamashita S, Nagataki S, Kondo T