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Step 2: Be consistent

This slowed emptying, combined with changes in bile metabolism such as lower bile acid production and increased excretion creates conditions that make gallstone formation more likely

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The lysate was centrifuged at 13,200 rpm for 10 min at 4C, and cleared lysate was used to determine the amount of GSH in the sample using the QuantiChrom glutathione assay kit (BioAssay Systems)