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qPCR assays SPHKAP knock-down efficiency Mouse islets transduced with either Control or SPHKAP shRNA lentiviral particles were lysed using Trizol and RNA extracted using 200 L chloroform per 1 mL Trizol and then precipitated in 500 L isopropanol and 5 mg glycogen per mL of Trizol

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IGF-1 LR3 carries an arginine substitution at position 3 and an N-terminal 13-amino-acid extension that reduces IGFBP affinity by roughly 1,000-fold, extending its active window to 20-30 hours

PLoS One 13 , e0199987 (2018)

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