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This fundamental metabolic failure is a primary driver of post-exertional malaise (PEM), the hallmark symptom of ME/CFS where even minor exertion triggers a severe exacerbation of systemic symptoms

We verified the impact of ACTL6A on GSH synthesis and confirmed that ACTL6A KD led to reduced expression of certain genes (GCLC, GPX2, GPX4, SLC7A11, SLC1A5, GSS, GLS, etc.) involved in the GSH synthesis pathway (Supplementary Fig

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