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The additional step with vials is proper reconstitution if using lyophilized (freeze-dried) tirzepatide

Such encapsulation of metal ions in the protein interior might hinder their dissociation and exclude formation of ternary ligand exchange complexes leading to kinetical inertness of the CuCP complex at pH 7.4

Loss of GPX4 protein expression in NRF1-deficient cells sensitized cells to ferroptosis, whereas in cells lacking GPX4, overexpression of NRF1 failed to inhibit ferroptosis

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