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It turns out that many researchers who did those studies were using the wrong cell lines, meaning that what seemed like a promising finding in kidney tumors was in fact a finding in cervical cancer

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After adding EnVision Flex 3,3-diaminobenzidine tetrahydrochloride (DAB, K346811-2, Agilent, Santa Clara, CA, USA) for 5 min at room temperature to visualize GPx3, the slides were washed with tap water, counterstained with hematoxylin and mounted in Pertex

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