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To detect Halo7-Rac1 in the EV-treated PANC-1 cells, blotted PVDF membranes were blocked in 5% (wt/vol) of ECL PRIME blocking reagent (Cytiva, #RPN418) in PBS-T overnight at 4 C and incubated with antibodies against Rac1, Halo or -actin in PBS-T containing 0.5% (wt/vol) ECL PRIME blocking reagent overnight at 4 C