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Calibration plots confirmed the model was well calibrated in the test set (Extended Data Fig
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CD8 + T cells expressing CD69 and granzyme B, which are indicative of cell activation after recent antigen exposure, are found adjacent to neurons infected with HSV-1 ( + T cells has been further illustrated in a study in which the supplementation of infected TG cultures with exogenous CD8 + T cells delayed the reactivation of HSV-1, and on the contrary, when these cultures were treated with an anti-CD8 antibody, which blocks the function of these cells, they displayed an increase in viral reactivation ( Furthermore, it has been reported that CD8 + T cell lytic granules are required for blocking the HSV-1 replication cycle and that granzyme B (GrB) cleaves the viral protein ICP4, which is needed for the transcription of early and late viral genes ( Interestingly, PD-L1 expression, a T-cell marker that binds to co-stimulatory molecules such as CD80, was also downregulated in latently-infected TG when infected with an ICP22 null virus ( + T cells induces HSV-1 reactivation in stressed mice through a regulatory effect that is mediated by Treg cells, which in turn allows the expression of viral genes, such as ICP0 and ICP4 ( It is important to note that more studies focused on the regulation of the immune response by neuronal signals are needed, namely on the characteristics of resident CD8 + T cells and other immune cells that surround the infected neurons in the TG
