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(5) Protein pellets were resuspended and vortexed in 250 L fresh RIPA buffer (150 mM NaCl, 1% Igepal CA-630, 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris, pH 8.0) with 0.1 mM PMSF, following which they were incubated at room temp for 5 min

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During clinical trials for those conditions, researchers noticed an interesting side effect: patients kept losing weight

Larger formats are available, but the 10mL size minimizes waste and improves protocol consistency for most labs

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Effect of choline supplementation on rapid weight loss and biochemical variables among female taekwondo and judo athletes. Journal of human kinetics 40 (2014): 77