The compounds in the low-molecular-weight fraction of the plasma from portal vein, which was collected 60 min after the ingestion of glutathione, were fractionated by size exclusion chromatography (SEC)
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Samples were then post-fixed in 1.0% osmium tetroxide, dehydrated in increasing concentrations of ethanol, then dehydrated at 31.1 C and 1,072 p.s.i
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There are 21 amino acids that are used to make proteins