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Plant J 37(1):2133

Effect of the NSAID nimesulide on the radical scavenger glutathione S-transferase in patients with osteoarthritis of the knee

Proteins were reduced with 5 mM dithiothreitol (Sigma-Aldrich) for 1 h at RT and alkylated with 10 mM iodoacetamide (Sigma-Aldrich) in the dark for an additional 45 min at RT

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The stereochemistry of the cysteine needs to be in the L- configuration to maintain the antioxidation cycle of the GSH analogues