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The third aliquot directly measures the combined pool of GSH and GSSG using previously published protocol with Ellmans reagent as a reporter

GSH and Aging GLUTATHIONE AND AGING--METABOLISM AND LIFE SPAN STUDIES 2014 Abstract Text: DESCRIPTION (Adapted from the Applicant's Abstract): Glutathione (GSH) is a major antioxidant present in high concentrations in nearly all living cells, with numerous critical functions in cellular homeostasis and defense

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A randomized cross-over clinical trial [127] included 33 men and 34 women who ate four different diets each for 14 days: one that was vegetable-free (basal), the basal diet supplemented with either a single or double dose of crucifers, and single dose cruciferous plus apiaceous vegetables at a level that was standardized to kilogram body weight

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