For Oil Red O Staining, liver tissues were frozen in Tissue-Tek CRYO-OCT (Fisher Scientific) and cryostat tissue sections were cut at 7 m thicknesses, fixed with 10% formalin for 10 min, and stained following an Oil Red O procedure
Diabetes 63 , 1224 (2014)
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The body has protective mechanisms against large energy deficits that make outrunning a plateau nearly impossible through exercise alone