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Blakesley VA, Butler AA, Koval AP, Okubo Y, LeRoith D: IGF-1 receptor function: transducing the igf-1 signal into intracellular events

Short-term: room temperature is fine, briefly Dry powder tolerates room temperature for days to weeks, such as during shipping

If necessary, keep the supernatant for analysis

This method has its pros and cons, while being helpful to measure cytokines potentially at a more localized level, these PBMCs can be affected by cryopreservation, length of storage, and the overall processing methods

Further insights from the study suggest that MGF might influence the cell cycle by potentially halting it at specific stages, and it may also activate the Mitogen-Activated Protein Kinase/Extracellular Signal-Regulated Kinase 1/2 (MAPK-Erk1/2) signaling pathway