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Cells were cultured for 4 days with or without hemin (1 M) and pyruvate (1 mM)
As mentioned previously, NRF2 regulates the expression of ATF4, which controls the expression of the glutamine transporter SLC1A5 [73], and asparagine synthetase (ASNS), which generates both asparagine and glutamate from aspartate and glutamine
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