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doi: 10.1001/jama.2020.4006 2 FitzGeraldOOgdieAChandranVCoatesLCKavanaughATillettWet al

Keep injecting, daily and for at least 90 days

Following incubation in serum-free medium containing 20 M DCFH-DA for 20 min at 37C, cells in each well were washed three times with PBS, digested by pancreatic enzymes at 37C for 3 min and immediately subjected to ROS measurement by flow cytometry analysis using Cytomics FC 500 MCL (Beckman Coulter, Inc., Brea, CA, USA)

The surprisingly downregulated expression of IL-1a in the diabetic group indicates impaired immunity against infection

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