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In cartilaginous fish (e.g., Callorhinchus milii , the elephant shark) the Gcg gene encodes 4 glucagon-like sequences, including a glucagon-like peptide 3 (GLP-3) (16), while in the frog Xenopus laevis (African clawed frog) the GLP-1 encoding exon has been triplicated to result in a coding sequence that could generate 5 glucagon-like sequences (17)