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While this principle has shown great promise in the research setting it has not yet come into routine use
To evaluate growth on solid media, in all cases -with the exception of the metE mutant, which only grew in the presence of B12 and, therefore, plating with and without B12 was performed from liquid cultures supplemented with B12- the liquid cultures grown without B12 were plated in solid 7H10-ADC plates supplemented with or without AdoCbl (10 g/mL) and/or L-Met (25 g/mL)
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Broadfield, L
CW Wong, FHKCP, FHKAM (Medicine) Department of Medicine and Geriatrics, Caritas Medical Centre, Shamshuipo, Hong Kong Corresponding author: Dr CW Wong ( Full paper in PDF [email protected] ) Abstract Vitamin B 12 deficiency is common among the elderly
