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Measurement of inflammatory markers Plasma inflammatory markers were measured using validated commercial assays for interleukin (IL)-18, IL-1, and IL-1 using Millipore Luminex Multiplex kit (Cat#HCYTA-60 K-03) and ELISA for vascular endothelial growth factor (VEGF) (ThermoFisher, Cat#KHG0111) and angiopoietin-2 (ThermoFisher, Cat#KHC1641)
Histone Modifications The histones can be modified in many ways posttranslationally, including acetylation, methylation, citrullination, ubiquitination, phosphorylation, and sumoylation (299, 300)
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