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Journal of Medicinal Food

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Cells were grown in DMEM/F12 supplemented with 10% fetal bovine serum, 100 g/mL penicillin/streptomycin/gentamicin (Invitrogen, Gibco) and in the condition of 95% air and 5% CO 2 humidified atmosphere at 37 C

In those who require 20 h of acetylcysteine, 2 h before completion of acetylcysteine infusion check ALT/AST and paracetamol concentration (in those with high initial paracetamol concentrations greater than double the 150mg at 4 h nomogram line) to determine need for ongoing acetylcysteine

And I think some animal studies by that same group have shown that