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Quantitative real-time PCR (qRT-PCR) analysis The total RNA was obtained from the cells lysed with Trizol reagent (Thermo Fisher, 15596-02, Waltham, MA)

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Efpeglenatide is composed of a modified exendin molecule attached to a fragment of human immunoglobulin 4 using a special technology called long-acting peptide/protein [49]