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In catalase and peroxidase, analysis of the crystal structure indicates strongly that amino-acid side chains are situated to aid in the cleavage by stabilizing a charge-separated transition state (Figure 5.14)

Nat Rev Neurosci 2015;16:358-72

A 20-nucleotide segment before NGG in the exon of LtGPX3 was selected, and amplified using primers modified with sticky ends (5 end) for single-guide RNA (sgRNA), which was inserted into the linearized pmCas9 vector (Fig