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Standard Dose 200-600 mcg per day via subcutaneous injection, or 1-2 mg two to three times per week

Droplet volume was maintained during freeze-thaw cycles using a constant flow of humidified nitrogen generated by combining humid nitrogen (0.010.05 L min 1 ) from a bubbler containing ultrapure water with dry nitrogen gas (0.6 L min 1 ) and passed through a mixing chamber before entering the cooling stage

In addition, a MEDLINE search using Medical Subject Headings (MeSH) was conducted

It means the most rapid improvement occurs early , with continued but more gradual benefit thereafter

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BT474 cells and PC3- hTERT cells were cultured in DMEM F-12 medium (Gibco, Invitrogen) with 10% FBS (Gibco, Invitrogen) and 1% PenicillinStreptomycin (Gibco)