Isolation of WAT stromal vascular cells and adipocyte differentiation To isolate the stromal vascular fraction, minced iWAT (combined depots from 45 mice per genotype) from 46-week-old mice was digested in 1 mg ml 1 collagenase D (Roche) with 1.5% BSA in sterile Hanks Balanced Salt Solution and incubated in a 37 C shaking water bath for 1 h
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It consists of a specific sequence of amino acids that primarily targets fat loss by enhancing the breakdown of fat and inhibiting lipogenesis
(5) Laferrre B, Hart AB, Bowers CY
Am J Ophthalmol 36:16771685 Blomstrand R, Ingemansson SO (1984) Studies on the effect of 4-methylpyrazole on methanol poisoning using the monkey as an animal model: with particular reference to the ocular toxicity